The OVA featured the previously unanimated "Mad Wax" arc from the manga. Bundled with a limited edition of the manga's 20th volume.

Layton receives a letter from the famed opera singer, Jenis Quatlane, inviting them to see her newest performance. She also asks Layton, Luke and Remi for assistance in a mystery, saying her friend Melina died one year ago, but returned later as a seven year old girl saying she had found the secret of eternal life, and she also knew all the secrets that only the true Melina could know. Naturally, the case fascinates Layton and his apprentice, so they head to the Crown Petone opera house, but Remi stays in London, searching for some girls that went missing in there. The opera was about a lost kingdom, The Kingdom of Immortals; once, there was a beautiful queen that loved music. She was beloved by everyone in the kingdom. But one day, the queen became very ill. The people of the kingdom toiled to find a cure, but medicine couldn’t help. The queen finally passed away, and right after, the cure was completed: A potion for immortality. The people of the kingdom deeply lamented for the death of their queen. In order to the meet reincarnated queen again, they decided to become immortal, using the just-completed immortality nostrum. Abrosia's Kingdom is still somewhere in this world, waiting for its queen to return, and then, she will sing forever. After the spectacle finishes, a masked men appears, and proposes something to the audience: "Let's play a game"- he says- "The winner, will have eternal life. The losers, will have to die." It's up to Layton and Luke to enter in the game, and discover the secret of Eternal Life. (Source: Wikipedia)

Sixteen years have passed since a mysterious alien ship crashed on Earth. Graviton City, once leveled by the ship's crash, is now a bustling metropolis. Within the city's high school, superhuman high school student A-Ko Magami dukes it out with the diabolical genius B-Ko Daitokuji, who will stop at nothing to win the friendship of A-Ko's buddy C-Ko Kotobuki. Meanwhile, in outer space, another alien ship is on course towards Earth to find its planet's lost princess. (Source: ANN)

Three weeks after the incident that left Captain Napolipolita's ship balancing on top of Graviton City, A-Ko and the gang go on summer vacation. While A-Ko ponders losing some weight and B-Ko devises another plan to defeat her redheaded rival to win C-Ko, Napolipolita and Spy-D experience severe homesickness - begging for a way to return to their home planet. Meanwhile, Hikari Daitokuji - B-Ko's father and the CEO of the Daitokuji Financial Group - arms the local military with new mecha to attack Napolipolita's ship and obtain its advanced technology.

Three weeks after the incident that left Captain Napolipolita's ship balancing on top of Graviton City, A-Ko and the gang go on summer vacation. While A-Ko ponders losing some weight and B-Ko devises another plan to defeat her redheaded rival to win C-Ko, Napolipolita and Spy-D experience severe homesickness - begging for a way to return to their home planet. Meanwhile, Hikari Daitokuji - B-Ko's father and the CEO of the Daitokuji Financial Group - arms the local military with new mecha to attack Napolipolita's ship and obtain its advanced technology. (Source: ANN)

In the middle of another break from school, A-Ko dreams of finding the perfect boyfriend. Because of this, she and C-Ko get into an argument that leads to C-Ko running away and nearly getting hit by a motorcyclist named Kei. While working part-time at a fast-food restaurant to raise money for a party dress, A-Ko meets Kei and immediately falls in love with him. B-Ko happens to like him as well; however, both A-Ko and B-Ko are unaware that C-Ko is the apple of Kei's eyes. (Source: ANN)

In Iraq, a group of archaeologists discover ancient relics that prophecize the coming of a superior race and the end of all civilization. Meanwhile, back in Graviton City, as A-Ko and B-Ko's rivalry over Kei intensifies, Mr. Daitokuji sets up an arranged marriage between Miss Ayumi and Kei. Furious over the engagement, both A-Ko and B-Ko do whatever it takes to prevent the wedding from going through. Little do they know that another alien fleet is on its way towards Earth.

In Iraq, a group of archaeologists discover ancient relics that prophecize the coming of a superior race and the end of all civilization. Meanwhile, back in Graviton City, as A-Ko and B-Ko's rivalry over Kei intensifies, Mr. Daitokuji sets up an arranged marriage between Miss Ayumi and Kei. Furious over the engagement, both A-Ko and B-Ko do whatever it takes to prevent the wedding from going through. Little do they know that another alien fleet is on its way towards Earth. (Source: ANN)

A-ko and B-ko are bounty hunters in an untamed world. When a young heiress, C-ko, falls out of the sky and into their hair, they find themselves battling space pirates and galactic police. Turns out the high-pitched, hungry C-ko holds a secret—the spirit of an ancient evil—and is the center of a plot to destroy the universe!

When in kindergarten a boy gets involved in an accident that is horrible damaging his arm. Somehow the doctors manage to save it. Now, several years later, his arm seems to become the focus of strange events, as it turns out that it's more than just a normal arm. Because of that a secret organization is out to get hold of him and the power he possesses.

A boy gets involved in an accident when in kindergarten, horribly damaging his arm, but the doctors somehow manage to save it. Now, several years later, his arm seems to be becoming the focus of strange events as it turns out to be more than a normal arm. Meanwhile, a secret organizations is out to get hold of him and the power he possess.

The 2nd Chapter continues the story of Takatsuki Ryou and his companions quest to discover the secret of the mysterious A.R.M.S. they all possess and what the future may hold for them. They now know where the answers lie but to get to them they must fight a desperate fight of survival against a powerful military corporation that wants their secrets no matter what the cost.